Elisa Robotti 1 , Fabio Quasso 2 , Marcello Manfredi 3 , Fabio Gosetti 4 , Eleonora Mazzucco 5 , Ciro Isidoro 6 , Emilio Marengo 7
Affiliations
1 Department of Sciences and Technological Innovation, University of Piemonte Orientale, Viale Michel 11, 15121, Alessandria, Italy. Electronic address: elisa.robotti@uniupo.it.
2 Department of Sciences and Technological Innovation, University of Piemonte Orientale, Viale Michel 11, 15121, Alessandria, Italy. Electronic address: fq004@libero.it.
3 Department of Translational Medicine, University of Piemonte Orientale, Via Solaroli 17, 28100 Novara, Italy; Isalit srl, c/o IPAZIA, Corso Trieste 15/A, 28100 Novara, Italy. Electronic address: marcello.manfredi@uniupo.it.
4 Department of Earth and Environmental Sciences, University of Milano-Bicocca, Piazza Della Scienza 1, 20126, Milano, Italy. Electronic address: fabio.gosetti@unimib.it.
5 Isalit srl, c/o IPAZIA, Corso Trieste 15/A, 28100 Novara, Italy. Electronic address: eleonora.mazzucco@uniupo.it.
6 Department of Health Sciences, University of Piemonte Orientale, Via P. Solaroli 17, 28100, Novara, Italy. Electronic address: ciro.isidoro@med.uniupo.it.
7 Department of Sciences and Technological Innovation, University of Piemonte Orientale, Viale Michel 11, 15121, Alessandria, Italy. Electronic address: emilio.marengo@uniupo.it
PMID: 32853885
DOI: 10.1016/j.jtemb.2020.126608
J Trace Elem Med Biol. 2020 Dec;62:126608. doi: 10.1016/j.jtemb.2020.126608. Epub 2020 Aug 14.
Abstract
Background: Based on the medical history and laboratory analytical tests, a patient presenting symptoms compatible with Chronic Fatigue Syndrome was suspected of metal intoxication; therefore, a chelating therapy was attempted. In parallel, the profile of elemental excretion in urine was determined.
Methods: Chelation therapy by CaNa2EDTA was administered every two weeks and urine samples were routinely collected for 17 months. The samples were mineralized with HNO3 69 % and analyzed by Inductively-Coupled Plasma – Mass Spectrometry. Data were processed by multivariate statistical methods.
Results: Most of the toxic elements showed a peak of excretion in 12-24 h after EDTA administration, which returned to basal level by 24-36 h after the treatment. Yet, the excretion of some trace elements persisted in the urine collected 26 h after the treatment.
Conclusions: The analysis of excreted metals following the CaNa2EDTA infusion allowed to monitor dynamically the chelation therapy. The chelation therapy was effective in mobilizing and eliminating the principal heavy metals present from the body. However, since such clearance almost vanished 24 h after the treatment, a protocol with more frequent and low-dose administrations is advisable to improve the metal excretion.
Keywords: Chelation therapy; Elemental profile; ICP-MS; Metal intoxication; Multivariate data analysis; Urine.
